The SNP markers produced from this project will be presented as a series of potential panels, each panel addressing a different issue related to Acipenser identification, as described in the project background rationale. As part of a future project, these panels will be characterized in terms of the SNP allele frequencies and their resulting accuracy and power for identification. Based on the number of SNPs in each panel and likely downstream application, potential genotype technologies for screening each panel will be described.
In order to fully validate the SNPs and provide a robust statistical evaluation of their utility to discriminate species, populations and breeding sources, it will be necessary to screen (genotype) multiple populations for all potential SNPs. While such a comprhenesive screening is currently beyond the resources of SturSNiP, for the species identification component a pilot-study screen of the ten most promising species-diagnostic SNP markers for 24 individuals in each species (96 fish) will be performed. This should enable to fully resolve the question of species identification.
The output from this project will be delivered in an appropriate format for subsequent development of the project results into a forensic genetic framework. TRACE Wildlife Forensics Network, the principle investigator of SturSNiP, is providing advice to the JRC on how to best design any follow-up on the work.